DIY, Electronics, Inspiring, LEDs, photography

Webcam DIY Microscope

I have a fascination with DIY microscopes. I have been making microscopes with ball lens and laser pointer lens etc. When these lenses are coupled with the powerful smartphone cameras, they produce highly magnified images of microscopic objects. However, I come across a very interesting webcam microscope through Guadi labs. Basically, when we reverse the lens of the webcam it acts as a microscope. There are many versions of this microscope in the Gaudi labs website, from which I chose the laser cut version for its simplicity. The parts were cut in 2016 when I was in St Andrews, but now only they are assembled as I kept this project in cold due to other interesting projects. The only improvement, I have done is connecting LEDS of different colours to the webcam board in place if its original white LEDS. That way I am planning to excite many fluorescent proteins.

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Webcam Microscope front view

 

Below you can find the microscopic images of cells (~ 30um in length) taken with this webcam microscope. I also took microscopic images taken from smartphone based microscope with laser pointer lens (details will be in another post, see reference 2). Clearly, webcam gives large magnification but small field of view. On the other hand, laser pointer lens gives smaller magnification and large field of view. So these two DIY low cost systems can be handy for biological applications. In fact, I am making one of this microscope for my colleague to quickly screen drug injected cancer cells to know whether the drug has reached inside the cells or not. I will follow up the progress of that project in a future post. I must tell you that these microscopes are far better than the $15 usb microscope attachments that can be bought online.

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Microscopic image of cancer cells taken with webcam microcope under white LED illumination. The slide was stained with a blue dye.

Cells with orange illumination

Microscopic image of cancer cells taken with webcam microcope under orange LED illumination.

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Microscopic image of cancer cells taken with webcam microcope under red LED illumination.

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Microscopic image of cancer cells taken with smartphone based microscope with laser pointer lens under white LED illumination.

 

References:

http://hackteria.org/wiki/index.php/DIY_microscopy

http://www.instructables.com/id/10-Smartphone-to-digital-microscope-conversion/

Acknowledgments:

Dundee Makerspace

Vamsi

Uday

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ARDUINO, DIY, Electronics, Inspiring, LEDs, photography, Raspberry Pi, Robotics

Experiments with a Light Meter

Why I am interested in Light meters?

When I was working in Scotland, I came across photo dynamic therapy (PDT), which uses light sensitive drugs to kill cancer cells. In the entire UK, there are only two PDT centers (afaik), one of which is in Dundee. By visiting the PDT center in Dundee, I realised that after applying the PDT drugs, doctors ask patients to wait in  sun light for two hours. There is no particular reason for exactly two hours of exposure to sun light. Therefore, it is not possible to know how much light dose has been received by the patients. To address this problem, PDT center at Dundee measured sunlight across the UK and Ireland and suggested that  cheap lux meters can be used to measure the required lux dose. I met with one of the PI and discussed about this in detail.

The problem with cheap light meters:

However, most commercially available cheap lux meters can only give instantaneous measure of light. These are originally developed for photographers to know lighting in their photo and building mangers to know lighting in a room. But PDT application  requires the lux values to be logged, aggregated to know whether the required light dose is reached. I think the only way to realise that is through connecting the lux meters to a microcontroller and stream the values to a smartphone. For that I am going to use a cheap lux meter that I can confidently modify after reading this blog post .

What I did:

I ordered the lux meter with a brand name “Ceto”from the same vendor as suggested in the blog post mentioned above. I identified the pins required to tap to get the lux values out. These are the pins on the amplifier. I soldered wires to these pins to read the voltage. So effectively LUX values are converted to voltage values in this lux meter. For example LUX of 290 is converted as 0.288V. I connected these wires to a multimeter to  see these voltage values.

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Guts of the LUX meter

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Zooming inside

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Red wire is signal

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Black wire is ground

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Hot glue to keep the wires in place

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Made a hole to the case to let the soldered wires come out, so that I can feed them into a multimeter

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More hot glue to fix the wires to the case

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Connected the wires to multimeter and we can see the light values appearing on the Multimeter as voltage values.

In the next step, I will connect the lux meter to a Arduino Uno and Bluetooth so that its possible to record the  aggregated lux values overtime time to determine the light dose for PDT treatment. I will write these details in another post.

P.S: It is just one of my hobby project, not related to my research.

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Electronics, LEDs

Fancy Christmas Dress with LEDs

Recently, one of my friend told me that he has a fancy dress competition sort of thing in his office. We went  for shopping with a hope of finding a Christmas themed sweater with LEDs, with a hope of winning the prize. I remember seeing them in Primark in the UK, but now I moved to Ireland. In Ireland, Primark is labelled as Penneys, where we didn’t find any such fancy dress. So we bought a sweater, nonetheless with Christmas Trea on it. We decided to decorate that with LEDs. I have some LEDs lying around, which I bought from Dealz (which is Poundland in the UK). Somehow, I sewed the LEDS, but its not a neat job. But I managed to hide the ugly sewing job with another sewing job (see the pics). But the end product is very good.

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LEDs sewed on to Christmas Tree

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Blue and Yellow LEDs

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Only Blue LEDS

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Only Yellow LEDS

 

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Backside with jungle of wires

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Close-up of entangled wires

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Wires covered with a napkin

 

My friend won the fancy dress prize, not because he was the only one to wear a sweater with LEDs, but others have just bought their dresses with LEDs already built into them. Moreover, their LEDs were quite low power ones, which can’t be seen in dark, where as ours is quite powerful. Why not, we are powering with 4AA batteries versus their coin batteries.

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